Sequence Conservation of the rad21 Schizosaccharomyces pombe DNA Double-Strand Break Repair Gene in Human and Mouse
Identifieur interne : 003F27 ( Main/Exploration ); précédent : 003F26; suivant : 003F28Sequence Conservation of the rad21 Schizosaccharomyces pombe DNA Double-Strand Break Repair Gene in Human and Mouse
Auteurs : Michael J. Mckay [Pays-Bas] ; Christine Troelstra [Pays-Bas] ; Peter Van Der [Pays-Bas] ; Roland Kanaar [Pays-Bas] ; Bep Smit [Pays-Bas] ; Anne Hagemeijer [Pays-Bas] ; Dirk Bootsma [Pays-Bas] ; Jan H. J. Hoeijmakers [Pays-Bas]Source :
- Genomics [ 0888-7543 ] ; 1996.
English descriptors
- Teeft :
- Academic press, Accession, Amino, Amino acid sequence, Amino acids, Basic residues, Biol, Birkenbihl, Bootsma, Cdna, Cell cycle, Cell cycle regulation, Cell cycle stage, Cellular processing, Cerevisiae, Checkpoint, Chromosomal, Chromosomal aberrations, Chromosomal localization, Chromosome, Database, Double strand, Dsbs, Elegans, Elegans cdna, Elegans probe, Genbank accession, Gene, Gene product, Genom, Genomic, Genomic instability, Genomic sequence, Genomics, Gnma, Hela, Hela cells, Hoeijmakers, Homolog, Homologs, Homology, Human homolog, Hybridization, Jeggo, Kinase, Localization, Mammalian, Mammalian mutants, Mammalian proteins, Mckay, Meiotic recombination, Mouse homolog, Mrna, Mrna expression, Mutant, Mutation, Northern blot analysis, Nucleotide, Other species, Phosphorylation, Phylogenetic, Pombe, Possible role, Protein kinase, Putative, Recombination, Repair gene, Saccharomyces, Saccharomyces cerevisiae, Schizosaccharomyces, Schizosaccharomyces pombe, Ssion yeast, Structural homologs, Subramani, Testis, Testis tissue, Thymidine, Transcript, Yeast.
Abstract
Abstract: Therad21gene ofSchizosaccharomyces pombeis involved in the repair of ionizing radiation-induced DNA double-strand breaks. The isolation of mouse and human putative homologs ofrad21is reported here. Alignment of the predicted amino acid sequence of Rad21 with the mammalian proteins showed that the similarity was distributed across the length of the proteins, with more highly conserved regions at both termini. The mHR21sp(mouse homolog of Rad21,S. pombe) and hHR21sp(human homolog of Rad21,S. pombe) predicted proteins were 96% identical, whereas the human andS. pombeproteins were 25% identical and 47% similar. RNA blot analysis showed thatmHR21spmRNA was abundant in all adult mouse tissues examined, with highest expression in testis and thymus. In addition to a 3.1-kb constitutive mRNA transcript, a 2.2-kb transcript was present at a high level in postmeiotic spermatids, while expression of the 3.1-kb mRNA in testis was confined to the meiotic compartment.hHR21spmRNA was cell cycle regulated in human cells, increasing in late S phase to a peak in G2 phase. The level ofhHR21sptranscripts was not altered by exposure of normal diploid fibroblasts to 10 Gy ionizing radiation.In situhybridization showed thatmHR21spresided on chromosome 15D3, whereashHR21splocalized to the syntenic 8q24 region. Elevated expression ofmHR21spin testis and thymus supports a possible role for therad21mammalian homologs in V(D)J and meiotic recombination, respectively. Cell cycle regulation ofrad21,retained fromS. pombeto human, is consistent with a conservation of function betweenS. pombeand humanrad21genes.
Url:
DOI: 10.1006/geno.1996.0466
Affiliations:
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Mckay</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>MGC Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000DR, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Troelstra, Christine" sort="Troelstra, Christine" uniqKey="Troelstra C" first="Christine" last="Troelstra">Christine Troelstra</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>MGC Department of Cell Biology and Genetics, Erasmus University, P.O. 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Box 1738, 3000DR, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Smit, Bep" sort="Smit, Bep" uniqKey="Smit B" first="Bep" last="Smit">Bep Smit</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>MGC Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000DR, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Hagemeijer, Anne" sort="Hagemeijer, Anne" uniqKey="Hagemeijer A" first="Anne" last="Hagemeijer">Anne Hagemeijer</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>MGC Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000DR, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Bootsma, Dirk" sort="Bootsma, Dirk" uniqKey="Bootsma D" first="Dirk" last="Bootsma">Dirk Bootsma</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>MGC Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000DR, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Hoeijmakers, Jan H J" sort="Hoeijmakers, Jan H J" uniqKey="Hoeijmakers J" first="Jan H. J." last="Hoeijmakers">Jan H. J. Hoeijmakers</name><affiliation wicri:level="3"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>MGC Department of Cell Biology and Genetics, Erasmus University, P.O. Box 1738, 3000DR, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Genomics</title><title level="j" type="abbrev">YGENO</title><idno type="ISSN">0888-7543</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="305">305</biblScope><biblScope unit="page" to="315">315</biblScope></imprint><idno type="ISSN">0888-7543</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0888-7543</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Accession</term><term>Amino</term><term>Amino acid sequence</term><term>Amino acids</term><term>Basic residues</term><term>Biol</term><term>Birkenbihl</term><term>Bootsma</term><term>Cdna</term><term>Cell cycle</term><term>Cell cycle regulation</term><term>Cell cycle stage</term><term>Cellular processing</term><term>Cerevisiae</term><term>Checkpoint</term><term>Chromosomal</term><term>Chromosomal aberrations</term><term>Chromosomal localization</term><term>Chromosome</term><term>Database</term><term>Double strand</term><term>Dsbs</term><term>Elegans</term><term>Elegans cdna</term><term>Elegans probe</term><term>Genbank accession</term><term>Gene</term><term>Gene product</term><term>Genom</term><term>Genomic</term><term>Genomic instability</term><term>Genomic sequence</term><term>Genomics</term><term>Gnma</term><term>Hela</term><term>Hela cells</term><term>Hoeijmakers</term><term>Homolog</term><term>Homologs</term><term>Homology</term><term>Human homolog</term><term>Hybridization</term><term>Jeggo</term><term>Kinase</term><term>Localization</term><term>Mammalian</term><term>Mammalian mutants</term><term>Mammalian proteins</term><term>Mckay</term><term>Meiotic recombination</term><term>Mouse homolog</term><term>Mrna</term><term>Mrna expression</term><term>Mutant</term><term>Mutation</term><term>Northern blot analysis</term><term>Nucleotide</term><term>Other species</term><term>Phosphorylation</term><term>Phylogenetic</term><term>Pombe</term><term>Possible role</term><term>Protein kinase</term><term>Putative</term><term>Recombination</term><term>Repair gene</term><term>Saccharomyces</term><term>Saccharomyces cerevisiae</term><term>Schizosaccharomyces</term><term>Schizosaccharomyces pombe</term><term>Ssion yeast</term><term>Structural homologs</term><term>Subramani</term><term>Testis</term><term>Testis tissue</term><term>Thymidine</term><term>Transcript</term><term>Yeast</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Therad21gene ofSchizosaccharomyces pombeis involved in the repair of ionizing radiation-induced DNA double-strand breaks. The isolation of mouse and human putative homologs ofrad21is reported here. Alignment of the predicted amino acid sequence of Rad21 with the mammalian proteins showed that the similarity was distributed across the length of the proteins, with more highly conserved regions at both termini. The mHR21sp(mouse homolog of Rad21,S. pombe) and hHR21sp(human homolog of Rad21,S. pombe) predicted proteins were 96% identical, whereas the human andS. pombeproteins were 25% identical and 47% similar. RNA blot analysis showed thatmHR21spmRNA was abundant in all adult mouse tissues examined, with highest expression in testis and thymus. In addition to a 3.1-kb constitutive mRNA transcript, a 2.2-kb transcript was present at a high level in postmeiotic spermatids, while expression of the 3.1-kb mRNA in testis was confined to the meiotic compartment.hHR21spmRNA was cell cycle regulated in human cells, increasing in late S phase to a peak in G2 phase. The level ofhHR21sptranscripts was not altered by exposure of normal diploid fibroblasts to 10 Gy ionizing radiation.In situhybridization showed thatmHR21spresided on chromosome 15D3, whereashHR21splocalized to the syntenic 8q24 region. Elevated expression ofmHR21spin testis and thymus supports a possible role for therad21mammalian homologs in V(D)J and meiotic recombination, respectively. Cell cycle regulation ofrad21,retained fromS. pombeto human, is consistent with a conservation of function betweenS. pombeand humanrad21genes.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li></region><settlement><li>Rotterdam</li></settlement></list><tree><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Mckay, Michael J" sort="Mckay, Michael J" uniqKey="Mckay M" first="Michael J." last="Mckay">Michael J. Mckay</name></region><name sortKey="Bootsma, Dirk" sort="Bootsma, Dirk" uniqKey="Bootsma D" first="Dirk" last="Bootsma">Dirk Bootsma</name><name sortKey="Hagemeijer, Anne" sort="Hagemeijer, Anne" uniqKey="Hagemeijer A" first="Anne" last="Hagemeijer">Anne Hagemeijer</name><name sortKey="Hoeijmakers, Jan H J" sort="Hoeijmakers, Jan H J" uniqKey="Hoeijmakers J" first="Jan H. J." last="Hoeijmakers">Jan H. J. Hoeijmakers</name><name sortKey="Kanaar, Roland" sort="Kanaar, Roland" uniqKey="Kanaar R" first="Roland" last="Kanaar">Roland Kanaar</name><name sortKey="Smit, Bep" sort="Smit, Bep" uniqKey="Smit B" first="Bep" last="Smit">Bep Smit</name><name sortKey="Troelstra, Christine" sort="Troelstra, Christine" uniqKey="Troelstra C" first="Christine" last="Troelstra">Christine Troelstra</name><name sortKey="Van Der, Peter" sort="Van Der, Peter" uniqKey="Van Der P" first="Peter" last="Van Der">Peter Van Der</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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